Products

1. PZA DST Kit

Catalog No.: PZA2026V2S

The PZA DST Kit (PZA2026V2S) is a dry-format 96-well PZA MIC plate designed for quantitative determination of the minimum inhibitory concentration (MIC) of Pyrazinamide (PZA) against Mycobacterium tuberculosis isolates at neutral pH (6.8) using standardized broth microdilution method (BMM).

Scientific Rationale

The testing is based on the standardized CLSI BMM format, which is well established for susceptibility testing of Rifampin, Isoniazid, and other anti-tuberculosis agents and antibiotics. 
In contrast, conventional PZA susceptibility testing requires acidic conditions and inocula up to 10-fold higher than the standard DST inoculum range. These non-optimal conditions have been associated with documented false results, including detection a 10% resistant population rather than the standard 1% proportion that correlates with treatment response, as well as reduced assay reproducibility.
The neutral pH (6.8) testing platform is designed to maintain conditions closer to optimal Mycobacterium tuberculosis growth, eliminate acid-mediated growth inhibition, enable testing within the standard CLSI inoculum range, and improve the reliability and reproducibility of quantitative PZA MIC determination.

Key Features

  • Simplified workflow
    • Standard incubation conditions using the CLSI-recommended inoculum range (2–8 × 10⁵ CFU/mL) with visible growth based result interpretation
    • Direct inoculum dilution in standard Middlebrook 7H9 broth (pH 6.8)
    • Results available within 7–21 days
    • On-scale QC range validation using reference Mycobacterium tuberculosis strains
  • Flexible, cost-efficient format
    • Individually packaged plates
    • Up to 8 isolates per plate
    • Suitable for small-batch testing with minimal waste
  • Extended stability
    • 12-month shelf life
    • 2-8°C storage
      For Research Use Only.

2. MycoGI PZA DST Kit

Catalog No.: PZA2026V2C

The MycoGI PZA DST Kit (PZA2026V2C) is a dry-format 96-well PZA MIC microdilution plate integrated with a mycobacterial growth indicator (MycoGI) fluorescence detection system. It is designed for quantitative determination of the minimum inhibitory concentration (MIC) of pyrazinamide (PZA) against Mycobacterium tuberculosis isolates at neutral pH (6.8) using the standardized broth microdilution method (BMM).

The assay incorporates an oxygen-quenched fluorescence sensor to monitor mycobacterial growth. During bacterial replication, oxygen consumption relieves quenching of a silicone-embedded fluorescent sensor positioned at the bottom of each well. Upon excitation with UV light, the sensor emits fluorescence proportional to bacterial growth, enabling clear, objective MIC endpoint determination and facilitating earlier result readout. 

Scientific Rationale

This assay leverages the standardized CLSI broth microdilution methodology widely used for antimicrobial susceptibility testing, including most first-line anti-tuberculosis agents.

In contrast, conventional PZA susceptibility testing requires acidic conditions and bacterial inocula up to ten-fold higher than the standard DST inoculum range. These non-optimal conditions have been associated with documented false results, including detection of a 10% resistant subpopulation rather than the conventional 1% proportion that correlates with clinical treatment response, as well as reduced assay reproducibility.

The neutral pH (6.8) platform supports testing under conditions closer to optimal Mycobacterium tuberculosis growth, mitigating acid-mediated growth inhibition while enabling use of the standard CLSI inoculum range.

In addition, the assay incorporates an oxygen-quenched fluorescence sensor to monitor mycobacterial growth. During bacterial replication, oxygen consumption relieves quenching of a silicone-embedded fluorescent sensor positioned at the bottom of each well. Upon excitation with UV light, the sensor emits fluorescence proportional to bacterial growth. This fluorescence-based growth indicator enhances signal clarity, reduces interference from cellular debris or media precipitates, and improves the objectivity, interpretability, and reproducibility of quantitative PZA MIC determination, while facilitating earlier result readout.

Key Features

  • Built-in MycoGI fluorescent growth indicator
    • Clear visual fluorescence endpoint
    • Minimizes interference from Pinpoint artifacts at the bottom of wells:
      • Dead bacterial debris
      • Media precipitation
    • Compatible with automatic fluorescence reading
  • Simplified setup
    • Direct inoculum dilution in standard Middlebrook 7H9 broth (pH 6.8)
    • Standard incubation conditions using the CLSI-recommended inoculum range (2–8 × 10⁵ CFU/mL) with highly sensitive fluorescence-based result interpretation
    • Results available within 6–10 days
    • On-scale QC range validation using reference Mycobacterium tuberculosis strains
  • Flexible, cost-efficient format
    • Individually packaged plates
    • Up to 8 isolates per plate
    • Suitable for small-batch testing with minimal waste
  • Extended stability
    • 12-month shelf life
    • 2-8°C storage

For Research Use Only.

3. Manual Microplate Fluorescence Viewbox

Catalog No.: PZA2026MV

The Manual Microplate Fluorescence Viewbox is designed for simple and reliable visual reading of fluorescence-based MycoGI DST microplates.

Intended Use

For use with MycoGI DST products to facilitate manual fluorescence-based growth interpretation.

Description

A mirrored fluorescence viewing device optimized for manual interpretation of MycoGI DST plates, providing:

  • Enhanced visualization of fluorescent endpoints
  • Improved contrast and readability
  • Compact (6.3” W × 7” H × 7” L) and laboratory-friendly design